1.3.2 检测IGA型CIC 采用双抗体夹心法(另文报告)。即包被兔抗HRFS→病人血清(HFRSV-IGA抗体)→HRP抗人IGA。
1.3.3 检测IGE型CIC 采用捕获法。参见文献[7]。即包被兔抗人IGE→病人血清(IGE抗体-HFRSV)→HRP-抗HFRS。
2 结果
2.1 不同病期、临床型HFRS病人血清中特异性抗体及特异性CIC水平(几何平均滴度、GMT)及阳性率结果。见表2。
表2 不同病期HFRS病人血清特异性IGA、IGE抗体及其特异性
CIC的GMT值与阳性率测定结果
TABLE 2 THE RESULTS OF SPECIFIC IGA, IGE ANTIBODIES AND SPECIFIC CIRCULATING IMMUE COMPLEX IN DIFFERENT ILLNESS PHASES OF HFRS
抗体型别
TYPE OF
ANTIBODY 病期
PHASE OF
ILLNESS 病例数
NUMBERS OF CASE 轻型SLIGHT (POSITIVE RATE)(%) 中型MIDDLE (POSITIVE RATE)(%)
抗体SPECIFIC
ANTIBODY CIC ANTIGEN SPECIFIC 抗体SPECIFIC
ANTIBODY CIC ANTIGEN SPECIFIC
发热FEVER 38 104.76(100.00) 11.41(55.56) 34.34(83.33) 3.21(61.11)
休克少尿 14 320.00(1/1) 80.00(1/1) 95.14(100.00) 17.84(87.50)
SHOCK & OILGURIA
IGA型 多尿POLYURIA 25 246.75(100.00) 21.87(62.50) 185.62(100.00) 30.25(85.71)
恢复RECOVERY 31 367.58(100.00) 28.99(80.00) 213.58(100.00) 11.48(58.33)
综算TOTAL 108 204.88(100.00) 18.88(66.67) 96.43(94.23) 10.25(63.46)
发热FEVER 35 29.58(81.25) 11.76(56.25) 57.81(88.24) 15.28(70.59)
休克少尿 12 - - 114.95(100.00) 12.63(71.43)
SHOCK & OILGURIA
IGE型 多尿POLYURIA 20 100.80(100.00) 7.10(50.00) 249.99(100.00) 15.26(63.64)
恢复RECOVERY 26 269.12(100.00) 5.53(41.67) 386.53(100.00) 7.48(54.55)
综算TOTAL 93 80.06(91.18) 8.24(50.00) 148.64(95.65) 12.51(67.39)